The Laundry Room box is the first of the run, and it already holds what runs 1 and 2 unlocked. Take the Circular Saw (it needs no ammo and tears through bosses), both defense books (The Secrets of Defense and The Essence of Defense, which cut the damage you take while blocking) and the Albert-01R handgun. The X-Ray Glasses, if they're there, keep the item-spotting effect on all the time and make coins far easier to see; take the Walking Shoes too if you have them. Most of these only work while carried.